Figure 2
SEC-MALS. The molecular weight of the FnCpf1–crRNA–DNA complex was determined by SEC-MALS-RI-UV. The Rayleigh ratio at 90° (LS 5; continuous line), ultraviolet absorbance (UV; dashed line) and weight-average molar masses (MW) for the complex (red), protein (green) and nucleic acid (blue) are plotted versus the elution volume, showing constant molar-mass values over the entire peak width. |