Figure 3
Crystals of the ternary FnCpf1–crRNA–DNA complex. (a, b) Crystals of the FnCpf1–crRNA–DNA complex obtained in 0.35 M sodium thiocyanate, 20%(w/v) PEG 3350 (see §2) under visible (a) and UV (b) light, revealing the presence of protein. (c) Diffraction pattern from crystals of the FnCpf1–crRNA–DNA complex. (d) Enlargement of the diffraction image. High-resolution reflections extend to 3 Å. (e) An FnCpf1 complex crystal mounted on a CryoLoop for a diffraction experiment. |