 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 5]](nw5058fig5mag.jpg)
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Figure 5
SDS–PAGE analysis of dissolved crystal (lane 2) and the VLP solution used for crystallization (lane 1). Four crystals stored in liquid nitrogen for diffraction data collection were dissolved in 10 µl 20 mM MES pH 6.5 prior to SDS–PAGE performed using 5–20% gradient gels stained with Oriole fluorescent gel stain (Bio-Rad). The 57 kDa band is the full-length VP1 and the 50 kDa band is VP1 with the N-terminal arm digested. The source of the band near 37 kDa is not known; it is larger than both the S-domain and P-domain fragments. The 50 and 37 kDa bands in the crystallization solution were partly caused by VLP breakage owing to freezing and thawing. Lane M contains molecular-mass markers (labelled in kDa). |
![[Figure 5]](nw5058fig5.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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