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Figure 3
Summary of the results of a systematic buffer screening using differential scanning fluorimetry. Each pH/buffer was tested in triplicate, with either high salt (200 mM NaCl, green spots) or low salt (50 mM NaCl, yellow spots). The protein in the purification buffer (TBS) gave a Tm of 58.55 ± 0.33°C. The highest Tm of 64.25 ± 0.26°C was seen with 50 mM sodium citrate buffer pH 6 with 200 mM NaCl. The analysis of the melting curves was performed with the program Meltdown (Rosa et al., 2015BB25).

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 74| Part 1| January 2018| Pages 39-45
https://doi.org/10.1107/S2053230X17017708
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