Figure 1
Small-angle X-ray scattering from n-NmDsbD and c-NmDsbD. (a) Measured scattering data for n-NmDsbD (red) and c-NmDsbD (blue; scaled by a factor of 0.1 for clarity). Overlaid are the calculated scattering curves for n-EcDsbD (PDB entry 1l6p; black curve, χ2 = 2.38) and c-EcDsbD (PDB entry 1uc7; yellow curve, χ2 = 1.65). Inset: Guinier plots of the scattering data. (b) The pair-distance distribution functions for n-NmDsbD (red) and c-NmDsbD (blue). (c) Probable domain shapes for n-NmDsbD (SASBDB entry SASDCH7; red envelope, resolution = 19 ± 2 Å) and c-NmDsbD (SASBDB entry SASDCJ7; blue envelope, resolution = 18 ± 2 Å) obtained from ab intio modelling against the scattering data. The crystal structures of n-EcDsbD (PDB entry 1l6p) and c-EcDsbD (PDB entry 1uc7) are shown as cartoons. The grey envelopes represent the total volume encompassed by the 16 aligned models for n-NmDsbD (χ2 = 1.90 ± 0.01) and c-NmDsbD (χ2 = 1.60 ± 0.01). |