 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 5]](ow5002fig5mag.jpg)
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Figure 5
Unfolding transitions of the M2–1 RBD. (a) Far-UV CD spectra of the M2–1 RBD at increasing concentrations of GdmCl from 0 to 5 M, as indicated by the arrow. (b) RBD equilibrium unfolding transition followed by the molar ellipticity at 220 nm (empty circles) and tyrosine fluorescence at 310 nm (filled circles). Ellipticity data were fitted to a two-state unfolding model (see § ![[link]](../../../../../../logos/arrows/f_arr.gif) 2). Inset: comparison of GdmCl equilibrium unfolding of apo M2–1 (empty circles; Esperante et al., 2011) with the M2–1 RBD (filled circles) monitored by the changes in molar ellipticity at 220 nm. Experiments were carried out at pH 7.0 (see §2). |
![[Figure 5]](ow5002fig5.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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