Figure 5
(a) Circular dichroism steady-state wavelength spectra for oxidized (red) and reduced (purple) Ec-Trx (∼0.07 mM) in NMR buffer at 20°C. (b) The CD thermal melts for oxidized (red) and reduced (purple) Ec-Trx obtained by recording the ellipticity at 218 nm at 2°C intervals between 10 and 90°C. |