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Figure 3
Structure of MmGADL1 in solution determined by SAXS. (a) Raw SAXS data (dots) overlaid with GASBOR (pink) and SREFLEX (green) model fits, as well as the theoretical scattering curve calculated from the crystal structure using CRYSOL (red). (b) Guinier plot. (c) The dimensionless Kratky plot indicates that MmGADL1 is folded, with limited flexibility. The red dot indicates the theoretical position of the peak in a folded globular protein. (d) Distance distribution of MmGADL1. (e) The GASBOR model (orange) superimposed with the crystal structure of MmGADL1 indicates a much more elongated conformation in solution. (f) Superposition of the SREFLEX (blue/grey) and GASBOR (orange) models suggests conformational changes relative to the crystal structure. (g) Comparison of the `open' conformation of DDC (orange/yellow; Giardina et al., 2011BB16) and the open conformation of MmGADL1 (blue/grey) seen here in solution.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 74| Part 1| January 2018| Pages 65-73
https://doi.org/10.1107/S2053230X17017848
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