Figure 2
SDS–PAGE of Met-hCCL5 purification. The SDS–PAGE recorded the individual steps of protein expression and purification: lane 1, before induction; lane 2, after induction; lane 3, supernatant from cell debris; lane 4, pellet from cell debris; lane 5, supernatant after refolding; lane 6, pellet after refolding; lane 7, target protein after HPLC purification. |