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Figure 1
SDS–PAGE and native PAGE analysis of purified wild-type CrPRX1 protein and its crystals. (a) SDS–PAGE showing the purification of CrPRX1 by Ni–NTA and gel-filtration chromatography. Lane 1, flowthrough from the nickel column. Lanes 2 and 3, fractions from the 20 mM imidazole wash. Lane 4, elution of CrPRX1 with 250 mM imidazole. Lanes 5 and 6, fractions from Superdex 200. (b) Native PAGE of CrPRX1 from dissolved crystals and solution samples. Lanes 1–3, CrPRX1 crystals. Lanes 4 and 5, CrPRX1 from stock protein solution.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 74| Part 2| February 2018| Pages 86-91
https://doi.org/10.1107/S2053230X17018507
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