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Figure 1
Electron-density differences induced by the reduction of bovine heart CcO are revealed by superposition of (|Foxi| − |Fred|) difference electron-density maps onto a CcO monomer. The metal cofactors, which contain iron, copper, magnesium and zinc, are displayed as spheres. Asp51 is shown in sticks and indicated by a black arrow. Pink sticks depict haems. (a) Difference map of the crystal obtained at pH 7.3 calculated at 1.99 Å resolution. The positive electron-density differences at the 6.0σ level (1.0σ = 0.2308 e Å−3) are shown in black. (b) Difference map of the crystal structure obtained at pH 5.7 calculated at 1.6 Å resolution. Positive electron-density differences at the 7.0σ level (1.0σ = 0.2700 e Å−3) are shown in black. The difference map for pH 7.3 shows smaller differences than that for pH 5.7. This is because the reduced crystal at pH 7.3 contains oxidized CcO in part as stated in the text.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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