Figure 4
Comparison of the PoFUT1 and PoFUT2 acceptor-binding sites. (a) Left: cartoon representation of MmPoFUT1 (cyan) in complex with MmEGF26 (salmon) and GDP (depicted as sticks with orange C atoms; PDB entry 5ky4; Li et al., 2017). Right: close-up view of the EFG binding site. The interacting amino acids of MmPoFUT1 and MmEGF26 are shown as sticks with cyan and salmon C atoms, respectively. (b) Left: cartoon representation of CePoFUT2 (slate blue) in complex with HsTSR1 (magenta) and GDP (sticks with orange C atoms; PDB entry 5foe; Valero-González et al., 2016). Upper right box: close-up view of the TSR binding site. C atoms of interacting residues are shown as sticks in slate blue (CePoFUT2) and magenta (HsTSR1). Lower right box: close-up view of the TSR binding site rotated 95° around the z axis and 35° around the y axis. Amino-acid colours are the same as in the upper panel and water molecules are shown as red spheres. |