view article

Figure 1
Hydrolysis of fluorogenic substrates by VC0615. (a) Michaelis–Menten kinetics for the hydrolysis of 4MU-β-D-glucose by VC0615. Error bars are standard deviations from three technical replicates. (b) Hydrolytic release of 4MU from various substrates. Only 4MU-β-D-glucose and 4MU-β-D-cellobiose show any hydrolysis. The release of 4MU from 4MU-β-D-cellobiose follows a lag period, consistent with processive hydrolysis by an exo-acting enzyme. (RFU, relative fluorescence units.)

ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds