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Figure 1
Hydrolysis of fluorogenic substrates by VC0615. (a) Michaelis–Menten kinetics for the hydrolysis of 4MU-β-D-glucose by VC0615. Error bars are standard deviations from three technical replicates. (b) Hydrolytic release of 4MU from various substrates. Only 4MU-β-D-glucose and 4MU-β-D-cellobiose show any hydrolysis. The release of 4MU from 4MU-β-D-cellobiose follows a lag period, consistent with processive hydrolysis by an exo-acting enzyme. (RFU, relative fluorescence units.)

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 74| Part 8| August 2018| Pages 512-523
https://doi.org/10.1107/S2053230X18011019
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