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Figure 2
TLC analysis of cello-oligosaccharide and chito-oligosaccharide substrate hydrolysis by VC0615. (a) VC0615 digestion of cello-oligosaccharides effects the gradual release of glucose and shorter oligosaccharides, consistent with the activity of a processive exo-glycosidase. (b) VC0615 effects the complete digestion of chitobiose within 30 min, compared with the relatively slow hydrolysis observed for the cello-oligosaccharides. (c) VC0615 does not hydrolyse N,N′-diacetylchitobiose. In all TLCs, the purple spot corresponds to citrate from the McIlvaine reaction buffer, which was not present in the standards. The citrate causes some displacement of lower spots. (d.p., degree of polymerization.)

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 74| Part 8| August 2018| Pages 512-523
https://doi.org/10.1107/S2053230X18011019
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