Figure 1
An example of incorrect modelling of N-glycosylation in the structure of the laccase McoG (PDB entry 5lm8; Ferraroni et al., 2017). The first NAG residue is attached to the Oδ atom (OD1 in PDB nomenclature) of Asn103, rather than to the Nδ atom. The clear electron density (2mFo − DFc at 1.2σ; clipped around the residues for clarity) and the fact that the neighbouring N-glycosylation on Asn60 is correct suggest that this is a simple oversight. Errors of this type are automatically corrected in PDB-REDO by swapping the names of the Oδ and Nδ atoms and updating the linkage description accordingly. This figure was produced using CCP4mg (McNicholas et al., 2011). |