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Figure 2
Differential scanning fluorimetry is represented as normalized fluorescence as a function of temperature. Controls with protein only (GH124) and EDTA are compared with samples containing one equivalent of each metal, i.e. calcium, copper, iron, manganese or nickel. Both controls and the sample with calcium show a maximum in fluorescence at ∼72°C. The melting temperature is shifted to 74–75°C in the presence of transition metals.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 74| Part 8| August 2018| Pages 496-505
https://doi.org/10.1107/S2053230X18006842
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