view article

Figure 2
Differential scanning fluorimetry is represented as normalized fluorescence as a function of temperature. Controls with protein only (GH124) and EDTA are compared with samples containing one equivalent of each metal, i.e. calcium, copper, iron, manganese or nickel. Both controls and the sample with calcium show a maximum in fluorescence at ∼72°C. The melting temperature is shifted to 74–75°C in the presence of transition metals.

ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds