 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](rl5163fig2mag.jpg)
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Figure 2
(a) The self-rotation function for B.c TssA CTD crystals calculated on all data from 55.90 to 3.79 Å resolution using a 44 Å radius of integration. The section shown corresponds to κ = 180°, with the orthogonal x, y and z axes along the crystallographic a, b and c axes, respectively. Noncrystallographic twofold axes can be seen every 11.25° in the bc plane at approximately 70% of the origin. The image was generated in POLARRFN (Winn et al., 2011  ). (b) Gel-filtration chromatogram of B.c SeMet-MBP-TssA CTD protein, with UV absorption (280 nm) shown as a dotted blue line and the elution volume (70.77 ml) corresponding to B.c TssA CTD shown above the curve. Inset: calibration curve for the gel-filtration column, with Kav = 0.4 (Ve = 70.77 ml) highlighted corresponding to a molecular mass of ∼3.55 × 105 Da for B.c TssA CTD. |
![[Figure 2]](rl5163fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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