Figure 2
Similarities between binding paratopes in DARPins off7 and 16. (a) Amino-acid sequence alignment between DARPins off7 and 16. The hyphens denote the absence of a third designed repeat in DARPin 16. Randomized interaction residues are colored red. The six amino acids that form the conserved core of the binding paratope are underlined and their corresponding positions in the two amino-acid sequences are indicated by blue lines. (b) Schematic view of the structural alignment between DARPins off7 and 16, illustrating the incongruity of the N-terminal capping and designed repeats. (c) Ribbon representation of the structural alignment between DARPins off7 (green) and 16 (red). The six conserved residues that form the core of the binding paratopes at the interface with MBP are shown as sticks. |