Figure 2
Two example structures of proteins with a molecular mass of less than 100 kDa determined by SPA cryo-EM. The upper row shows (a) the density map of the 64 kDa hemoglobin at 3.2 Å resolution solved using a Volta phase plate (Khoshouei et al., 2017) and (b) a section of density near the heme group, while the bottom row shows (c) the map for the 93 kDa enzyme isocitrate dehydrogenase 1 and (d) the quality of the map, demonstrating side-chain density in an α-helical region (Merk et al., 2016). Parts (a) and (b) are reproduced from Khoshouei et al. (2017) under a Creative Commons Attribution 4.0 International License, and parts (c) and (d) are reproduced from Merk et al. (2016), with permission from Elsevier. |