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Figure 3
(a) Cartoon representing the role of ComJ. The nuclease ComI degrades incoming DNA unless it is bound by ComJ, which inactivates ComI and allows foreign DNA to enter the cell. (b) Chemical structures of crocagin and ComX (RO-E-2). The similar tryptophan modifications are highlighted in red. (c) Two independent MST measurements of CgnJ with crocagin A (green and red). Since saturation could not be reached (owing to compound solubility), the Kd is estimated to be 2.4 ± 0.8 mM. |