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Figure 1
Electrophoretic and chromatographic analyses of the purified GldA used for crystallization. (a) 12% SDS–PAGE of purified GldA. Lane A, protein markers (labelled in kDa); lane B, purified E. coli GldA protein. (b) Elution profiles of GldA (solid line) and molecular-weight markers (dotted line) using Superdex 200 gel-filtration chromatography. P1, bovine thyroglobulin (669 kDa); P2, horse apoferritin (443 kDa); P3, sweet potato β-amylase (200 kDa); P4, yeast alcohol dehydrogenase (150 kDa); P5, bovine serum albumin (66 kDa); P6, bovine carbonic anhydrase (29 kDa). The estimated molecular weight of GldA is 161 kDa. (c) E. coli GldA protein crystals produced by the vapor-diffusion method.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 75| Part 3| March 2019| Pages 176-183
https://doi.org/10.1107/S2053230X19000037
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