Figure 3
The active site of E. coli GldA. Glycerol (cyan sticks) is sandwiched between Zn2+ (gray sphere) and NAD+ (plum sticks) superimposed from PDB entry 1jq5. An enlarged view of the active site is shown with bound glycerol, Zn2+ and NAD+. Zinc is coordinated by two conserved histidines (His254 and His271) and an aspartate (Asp171). The O1 and O2 atoms of glycerol (stabilized by Phe245) serve as ligands of the Zn2+ ion. |