Figure 2
The basic scattering functions of the large subunit of E. coli ribosomes from ASAXS at wavelengths near the K absorption edge of phosphorus. The upper curve is 〈|U(Q)|2〉 which does not depend on the wavelength. The lowest 〈|V(Q)|2〉 is the scattering intensity of the phosphorus atoms of the ribosomal RNA. The cross term 2〈Re{U(Q)V*(Q)}〉 is in the middle of the figure. The open spheres are the values of the basic scattering functions deduced from seven scattering functions measured at wavelengths near the K-edge of phosphorus. The line is obtained from an indirect method using the anomalous dispersion of the distance distribution function. There are about 1500 P atoms per 1500 kD of the ribosomal subunit. The intensity scale is given in arbitrary units. The data were obtained at the beamline A1 of HASYLAB (Hütsch, 1992). |