view article
Figure 8
Structure of Taq polymerase and normal mode analysis (NMA). Taq polymerase has two distinct domains: a 5′ nuclease (residues 1–289) and a Klentaq (residues 294–831) domain connected by a linker (residues 290–305). The Klentaq domain resembles a right hand with palm, finger and thumb subdomains. NMA on Taq polymerase using computer code ELNEMO (Suhre & Sanejouand, 2004BB19) can identify the type and direction of internal motions. The Klentaq domain can be subdivided into the 3′–5′ exonuclease domain (residues 294–422) and the polymerase domain (residues 424–831). The first six normal modes are the translational and rotational motions of Taq polymerase as a whole (not shown). The lowest internal seventh and eighth normal modes are relative en bloc motions between the Klentaq domain and the 5′ nuclease with 5′ nuclease moving towards the thumb, which is functionally important. Subdomain motions become apparent for the 9th, 10th and higher normal modes.

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 40| Part s1| January 2007| Pages s28-s33
doi:10.1107/S0021889806056093
Follow J. Appl. Cryst.
Sign up for e-alerts
E-alerts
Follow J. Appl. Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS