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![[Figure 2]](kk5065fig2mag.jpg)
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Figure 2
(a) Ca 2 µl of leftover protein solution and ca 2 µl of precipitant solution from the initial experiment (left) were used to generate an optimization experiment in the MPCS CrystalCard (right). In the CrystalCard, aqueous solutions (protein, precipitant and buffer) were combined and spontaneously segmented into individual drops (plugs) by the inert, immiscible carrier fluid. The resulting plugs filled the microcapillary and were incubated as individual crystallization experiments. Scale bar = 400 µm. (b), (c) Generic protein crystallization phase diagrams indicating how crystallization phase space is interrogated in MPCS optimizations. In Type 1 MPCS optimizations (b) protein concentration is held constant while a gradient of precipitant concentration is generated over a series of plugs. In Type 2 MPCS optimizations (c), protein concentration begins high and slowly decreases as precipitant concentration begins low and slowly increases to generate a dynamic protein versus precipitant gradient over a series of plugs. (d) A picture of an MPCS CrystalCard being peeled apart in order to expose the crystals. Scale bar = 1 inch ≃ 2.54 cm. (e) A picture of a protein crystal being harvested from a CrystalCard using a 0.2 mm cryo-loop. Scale bar = 200 µm. |
![[Figure 2]](kk5065fig2.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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