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![[Figure 4]](kk5077fig4mag.jpg)
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Figure 4
A table of DSF results for the proteins and PSBs. The numbers represent temperature shifts in K. Only 11 of the 20 protein samples gave useful DSF signals. PSB 93, hand soap, was removed as it was hydrophobic and significantly interfered with the DSF assay by interacting with the dye and causing unacceptably high background fluorescence, making the data uninterpretable. We used this position as a control, with only the protein and dye diluted with water loaded in that well. The temperature shift was calculated by reference to this control. In the case of proteins H, R and S a melting temperature could not be calculated from the control data. Blank areas in the table indicate that the melting data were difficult to impossible to interpret. In cases H, R and S, where we could not calculate a melting temperature of the protein in the absence of a PSB, the values in the table were calculated as a shift from the average value of the Tm for that protein for all of the interpretable Tm values of the protein with a PSB. Green and pink shaded cells denote temparature shifts ≥±2.0 K, respectively. |
![[Figure 4]](kk5077fig4.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
