Figure 2
Graphical display of intermediate data analysis. (a) Differential scanning fluorimetry (Tm) curve showing fluorescence intensity versus temperature; vertical bars are at transition midpoints (Tm) with a thicker bar for the major transition; horizontal bars are at FTm spanning the transition width (FWHM of the slope dF/dT). (b) Size-exclusion chromatography, absorbance in mA280 versus fraction, with superimposed models fitting multiple Gaussian peaks. (c) Dynamic light scattering histogram, plotted as intensity versus hydrodynamic radius. |