Figure 2
Data of one position on region (A) of the sample with B. thuringiensis cell material (cf. Table 1): (a) hologram Ih = I/Ie and (b) phase of holographic reconstruction which already yields a good impression of the sample structure. For instance, the rod-shaped bacteria (or their remnant empty cell envelopes) can be identified. However, the reconstruction appears distorted owing to the twin image problem. (c) The `radial' gradient of the holographic reconstruction in (b) used for estimating the support (cf. text). (d) Product of the final support and holographic phase reconstruction. Scale bars in (a) and in (b), (c), (d) denote 1 mm (real detector dimension) and 5 µm (effective geometry), respectively. |