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![[Figure 2]](vg5013fig2mag.jpg)
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Figure 2
SVD analysis and reconstruction of protein scattering from an SEC–SAXS experiment for Arf6. (a) Raw scattering data sets for Arf6NΔ13Q67L acquired in-line with chromatography on a 24 ml Superdex-200 column. (b) Raw and normalized total intensity elution profiles calculated by summing the intensities for each data set in (a) over the entire q range. Also shown are the incident (I0) and transmitted (I1) beam intensity profiles. (c) Singular values and autocorrelation of the columns of U and V after SVD of the data in (a). (d) Columns of the orthonormal U matrix corresponding to the rank-ordered singular values in (c). (e) Columns of the orthonormal V matrix multiplied by the corresponding rank-ordered singular values in (c). (f) CRYSOL fits of the theoretical scattering for the Arf6NΔ13Q67L-GTPγS crystal structure [PDB entry 2j5x (Pasqualato et al., 2001  ), with or without addition of a His6 tag from chain A in 2r09] to the protein scattering reconstructed by SVD–LC, Guinier-optimized subtraction or direct subtraction. |
![[Figure 2]](vg5013fig2.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
