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![[Figure 7]](ei5002fig7mag.jpg)
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Figure 7
The dialysis crystallization experiment with variation of the temperature using the integrated apparatus for optimization of crystallization in the case of chicken egg-white lysozyme. The scale represents 100 µm; images are ordered from left to right. (a) The first three images show the initial growth at 295 K with two different zooms. Then the crystal is shown at the beginning of temperature change to 291 and 288 K (zoom is not the same to fit the crystal size). The last image shows the crystal at the end of the crystal growth process at 285 K. (b) The first three images show the initial nucleation and crystal growth at 291 K. Then the temperature is increased to 313 K until total dissolution of all the crystals (next three images). The two last images show the second nucleation event and related crystal growth at 295 K. The precipitant concentration was kept constant at 0.9 M NaCl during the entire experiment. (c) The first four images show the initial growth at 0.9 M NaCl. Once the precipitant concentration has been lowered gradually to 0 M NaCl, replacing it with only Na acetate buffer at 0.1 M pH 4, successive dissolution of all the crystals is observed (next two images). The two last images show the second nucleation event and related crystal growth at 0.75 M NaCl. The temperature was kept constant at 291 K during the entire experiment. |
![[Figure 7]](ei5002fig7.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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