Figure 1
Schematic illustration of the experimental handling. The silicon chip is mounted directly on the beamline goniometer and positioned within a stream of humidified air. (a) A drop of 2–3 µl of crystal suspension is pipetted onto the upper side of the chip. (b) By attaching a wedge of filter paper to the bottom side of the chip, the mother liquor is then soaked through the pores while the crystals are retained by the chip. (c) Finally, the microcrystals are scanned through the primary beam. The diffraction pattern is recorded with a flat Pilatus 6M detector. Dehydration of the crystals is prevented by the humidified air. |