Figure 1
Distribution of the low counts in a typical MicroED image of proteinase K collected by continuous rotation using the rolling shutter mode of the camera. (a) The histogram in the second outermost shell between 1.5 and 1.7 Å for an uncorrected image, and (b) the histogram of the corresponding corrected image. The continuous curves in (b) show the fitted lognormal distributions in the two innermost (resolutions lower than 4.7 Å in blue, resolutions between 3.4 and 4.7 Å in orange) and the second outermost (black curve) resolution shells. As the resolution increases, the mode and the variance of the distribution decrease. |