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Figure 1
The GPA plot reveals the presence or absence of the Guinier region. (a) Scattering curve collected from a tetrameric maltose binding protein fusion protein (inset) (Mendillo et al., 2007BB10). (b) The traditional Guinier plot was linear, and the values Rg = 46.6 Å and I(0) = 6784.7 were extracted from the fit of the line (dashed) to the data (crosses) in the qRg range of 0.9–1.3. (c) The GPA plot reveals that a small number of points in the Guinier region (crosses) were collected, as shown by the presence of the observed rise in the GPA plot, even without fitting the Guinier approximation (dashed line). Deviations of experimental data at higher resolutions from the Guinier approximation correspond to regions for which the Guinier approximation no longer applies. (d) The peak in the dimensionless GPA plot for this sample has a value (1.396, 0.7420), which is close to the theoretical position of (1.5, 0.7428).

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