Figure 2
(a) SDS–PAGE of FtHAP purified according to the protocol described in the text. (b) Photograph of FtHAP crystals grown with the protocol used by the students. Crystals were grown as both hanging/sitting drops, from 2 µl of protein (10 mg ml−1) and 2 µl of well buffer solution, and typically appear overnight (see text). Crystals in this photograph (<0.5 mm) are representative of those used to collect the diffraction data in Table 2. Photograph by Kyle Stiers. |