Figure 3
Results of the SEC-SANS measurement of protonated Alba3 in 0% (a) and 100% (b) D2O buffer, showing the absorbance of the sample at 280 nm in blue and the averaged SANS intensity between Q = 0.012 Å−1 and Q = 0.041 Å−1 in red. (c) and (d) show individual SANS curves (30 s exposures) recorded during elution in 0 and 100% D2O buffer, respectively, from the top of the first (open circles) and second (full circles) peaks. The Rg values are obtained using the AutoRg function from primus (Konarev et al., 2003). |