Figure 3
Profilin:actin crystals and diffraction patterns. (a) Typical profilin:actin crystal with a diagonal cross along the 001 plane. (b) Normal periodic diffraction pattern (Porta & Borgstahl, 2012). (c) Incommensurately modulated diffraction pattern from crystal gMod3 with satellite reflections along b*. (d) Same as part (c) but with the main reflections circled in blue from the predicted positions calculated by the CrystalClear software (Rigaku, Tokyo, Japan). Note the satellite reflections are not predicted; only the main reflections are predicted. (e) Incommensurately modulated diffraction pattern from crystal xMod1 with satellite reflections in the b* direction. (f) Close-up of the modulated diffraction with approximated unit-cell dimensions. The blue circles are predicted reflection positions. Again, the satellite reflections are not predicted; only the main reflections are predicted. Images were collected at a distance of 300 mm with 15 min exposures and an oscillation of Δφ = 0.5°. Parts (a)–(c), (e) and (f) of this figure were reproduced from Lovelace et al. (2008). Note that the crystal diffraction data were processed with Eval15, not the CrystalClear software. CrystalClear was used here for the purposes of illustration only. |