Figure 5
(a) The bis-tris propane molecules (BTP) bound between the two monomers on each side of the biological tetramer in the structure of FabG from Cupriavidus taiwanensis solved at 1.6 Å resolution (PDB code 4nbv; Javidpour et al., 2014). (b) The crystal contact interface with a 2mFo–DFc electron density map contoured at 1.2σ is shown in blue around Asp107, Asp111, Lys115, Gln193 and the bis-tris propane molecule. Bis-tris propane stabilizes the charged residues Asp111 and Lys115 in one monomer and Asp107 in the other monomer. The crystal contact is coordinated by bis-tris propane and Gln193 from the symmetry-related copy. Contacts are shown as broken lines and distances in ångströms. |