Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Awel, S.; Kirian, R.A.; Wiedorn, M.O.; Beyerlein, K.R.; Roth, N.; Horke, D.A.; Oberthür, D.; Knoska, J.; Mariani, V.; Morgan, A.; Adriano, L.; Tolstikova, A.; Xavier, P.L.; Yefanov, O.; Aquila, A.; Barty, A.; Roy-Chowdhury, S.; Hunter, M.S.; James, D.; Robinson, J.S.; Weierstall, U.; Rode, A.V.; Bajt, S.; Küpper, J.; Chapman, H.N.

doi:10.1107/S1600576717018131

Journal of Applied Crystallography Journal of Applied
Crystallography

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Figure 2
Laboratory characterization of a beam of GV particles focused with the 15° convergent aerosol nozzle using a strong-magnification imaging microscope. (a) A single exposure, showing streaked images of GV particles caused by the 100 ns laser illumination. The particles are moving from left to right and their streaked images have non-uniform intensity due to the relatively slow decay of the illumination laser pulses. (b) The two-dimensional rate-corrected particle density determined from the centroids of individual particle images such as the one shown in (a). (c) Gaussian fit to the particle density at the focal plane in (b).

JOURNAL OF
APPLIED
CRYSTALLOGRAPHY

ISSN: 1600-5767

Volume 51| Part 1| February 2018| Pages 133-139

https://doi.org/10.1107/S1600576717018131

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