Figure 1
(a) The size-exclusion chromatography profile of MSP1E3D1:DMPC nanodiscs assembled under optimal stoichiometry and the scattering data from different fractions, as indicated by the vertical drop lines. Also plotted are the radii of gyration for each fraction (red symbols). The markers on top of the chromatogram denote the elution times of molecular weight standards (from left to right: the void volume, and 669, 443, 200, 150 and 66 kDa molecular masses). The flow rate was 1 ml min−1. (b) The scattering data, normalized for concentration. Data from fractions 55–60 min are coloured red, green, blue, cyan, magenta and orange, respectively. |