Figure 5
Difference WAXS changes in SoPIP2;1 associated with the release of caged Ca2+. (a) SAXS/WAXS absolute scattering before (black line) and after (green line) the sample was illuminated with UV, plotted as log[I(q)] versus log(q). (b) Experimental difference data qΔI(q), where ΔI(q) = I(q)UV − I(q)dark is shown in green. Data were collected for 7500 s with an X-ray exposure of 30 s per frame. A prediction (black line) based on known crystallographic structures (Törnroth-Horsefield et al., 2006) of the open and closed forms of SoPIP2;1 is shown for comparison. |