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Figure 4
Metrics for SAD structure determination for 50% MPD soaked thermolysin crystals. CC1/2-anom is for the low-resolution bin (∼30–8 Å) from AIMLESS (Karplus & Diederichs, 2015BB33; Evans, 2006BB9). FOM is the figure of merit from PHENIX using Autosol (Read & McCoy, 2011BB45; Adams et al., 2010BB1). Rfree is for the autobuilt model in PHENIX without any user intervention. Metrics are plotted against the overall redundancy of the data set to 2.0 Å, which corresponds to roughly 2× the anomalous redundancy. Blue dots = plunge cooled [protocol (3)]; solid orange = gas stream cooled [protocol (1)]; green dash = plunge cooled followed by rewarming and recooling in the gas stream [protocol (5)].

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