Figure 1
(a) The sampling areas for Raman and XRD analysis, showing a comparison of wild-type (WT) and hypophosphatemic (HYP) mouse tibia and fibula longitudinal cross sections embedded in (poly)methyl methacrylate blocks. The tibia are highlighted by dashed lines. (b) A schematic diagram of the tibia and fibula, showing the approximate positions of the Raman maps (stars) and 2D-XRD analysis (dashed circle). (c) A light microscopy image of a typical Raman map site (star) from a midshaft site on an HYP mouse bone. (d) A typical fit of a Raman spectrum taken from a WT mouse bone sample. |