Figure 4
FT–IR transmission maps of femoral bones from (a), (b) virgin WT mouse femoral bones and (c), (d) virgin HYP mice. Panels (a) and (c) show the mineral-to-collagen ratio measured using the collagen 1600–1700 cm−1 bands and the phosphate bands spanning 900–1200 cm−1, respectively. Panels (b) and (d) show the variability in carbonate content of the bone mineral phase using the carbonate and phosphate spectral bands spanning 1414–1424 and 900–1200 cm−1, respectively. Each map is 100 × 120 µm. |