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![[Figure 5]](gj5234fig5mag.jpg)
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Figure 5
The dialysis experiment with variation of the temperature in the case of crystal growth of EosFP from the process of LLPS. (a) Approximately 2 h after injection of the protein into the dialysis button at 293 K, the formation of the dense liquid droplets of the protein-rich phase emerging from the LLPS in the dialysis is observed. The temperature is increased to 295.5 K. Dissolution of the dense liquid droplets at 295.5 K (b) 30 s later, (c) 1 min later, (d) 90 s later and (e) 2 min later. (f) Approximately 6 h later at 295.5 K, the appearance of the first crystal formed as a result of the LLPS process is observed. Growth of the formed crystal at 295.5 K (g) 12 h later, (h) 36 h later, (i) 3 days later and (j) and 6 days later. (k) Schematic, temperature versus protein concentration, conceptual construct of the phase diagram incorporating some selected images (to be tracked in alphabetical order), showing the optimization workflow in growing EosFP crystals from the LLPS process. Various zones are shown: areas of the fluid (protein solution or F), coexistence of the protein-rich and protein-poor liquid phases (LLPS or L + L), coexistence of the crystal (S) and the protein solution (F + S), possible gelation (Gel), and optimum condition for protein crystallization (Optimal Crystallization). TC–CC represents the critical point beyond which LLPS is not longer possible. |
![[Figure 5]](gj5234fig5.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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