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Figure 7
Schematic phase diagrams (protein concentration versus temperature or precipitant concentration) incorporating some selected images (to be tracked in ascending order) and illustrating the crystallization optimization workflow in growing chicken egg-white lysozyme crystals: (a) Generating the large single lysozyme crystal obtained at constant chemical composition using the control of the temperature variations. (b) Generating uniformly sized lysozyme crystals at constant chemical compositions using the control of the temperature variations. (c) Generating uniformly sized lysozyme crystals at constant temperature using the control of the concentration of crystallizing agent. (d) Generating a large number of small uniformly sized lysozyme crystals obtained at constant chemical composition using the control of the temperature variations. The shrinkage of the metastable zone by increasing the rate generating the supersaturation is shown schematically. (Scale on photographs represents 100 µm.)

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 53| Part 3| June 2020| Pages 686-698
https://doi.org/10.1107/S1600576720003209
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