Figure 3
Securing the IMISX sachet in the holder clamp. (a), (b) A sachet containing a crystal-laden mesophase bolus removed from an IMISX crystallization plate is placed between and secured by the jaws of the clamp. (c), (d) Cartoon representation of the sachet in the clamp of an h1 and an h2 holder. The cubic phase bolus, the sponge phase bolus, the precipitant solution and crystals are shown in yellow, orange, blue and gray, respectively. (e), (f) Side view of the IMISX sachet secured in place between the jaws of the clamps in the h1 and h2 holders. The gap between the jaws of the clamp, marked with an arrowhead in (e), facilitates soaking of crystals with ligands, heavy atoms and wash solutions. |