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Figure 4
Procedures for soaking crystals in an IMISX sachet in 3D-printed holders. (a), (b) After the sachet has been mounted in the clamp as indicated in Fig. 3[link], the edges of the sachet are trimmed with a pair of scissors along the black dashed lines taking care not to cut across (open) the well. The well is opened by cutting along the dashed red line. The yellow disc in (a) and the orange random shape in (b) represent viscous mesophase and sponge phase boluses, respectively, in ideal positions in the windows of the clamp for data collection. (c), (d) Soak solution containing ligands, heavy atoms or wash solution is added to the bolus through the side or top openings in the sachet. (e), (f) View of the h1 and h2 holders mounted on the goniometer at the SLS beamline X06SA-PXI.

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