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![[Figure 2]](ei5059fig2mag.jpg)
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Figure 2
Effects of sample preparation procedures on the viability of infected High Five insect cells. (a) High Five cells imaged 4 days after infection with rBV HEX-1. Upper panel: differential interference contrast light microscopy and fluorescence microscopy of insect cells prior to sample preparation (u, untreated cells). EYFP fluorescence labelling of living, baculovirus-infected cells is shown in cyan. Lower panel: DIC and fluorescence microscopy of cells after sample preparation and propidium iodide (PI) staining (t, treated cells). PI fluorescence labelling of dead cells is shown in red. The scale bar applies to all panels. (b) Analysis of fractions of living, dead, uninfected and crystal-containing cells prior to and after sample preparation procedures within different High Five cell cultures. More than 60% of rBV-infected High Five cells are still vital after sample preparation and thus at the beginning of the diffraction experiment. The reduction of luciferase-crystal-containing cells is due to the instability of luciferase crystals outside the living cell. |
![[Figure 2]](ei5059fig2.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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