Figure 6
1D radially averaged X-ray scattering data of High Five insect cells containing intracellular crystals of the target proteins CatB (a) and HEX-1 (b) (SASBDB IDs: SASDH76 and SASDH66). The percentage of crystal-containing cells within the entire culture of each sample, as determined by light microscopy, is presented next to the scattering curves. The detection limit for in cellulo crystals using X-ray scattering at the P12 beamline setup 1 was determined to range between 0.3 and 6% of a crystal-containing cell fraction, depending on the respective protein. The insets show the scattering curve of the 16-fold diluted sample compared with that of mock-infected cells. |