view article
Figure 7
The unit-cell parameters of intracellular crystals do not depend on the insect cell line used for protein crystallization. Differential interference contrast light microscopy of intracellular CatB (a), (b) and HEX-1 (d), (e) crystals grown in Sf9 insect cells shows a highly comparable morphology compared with the growth in High Five cells. Red arrowheads highlight selected intracellular crystals. Corresponding X-ray scattering data show identical positions of the Bragg diffraction peaks in both cell lines for either CatB (SASBDB: SASDH96, SASDH86) (c) or HEX-1 (SASBDB: SASDHB6, SASDHA6) (f), indicating identical unit-cell parameters independent of the cell line used for crystal growth. The peak intensity is reduced in Sf9 cells owing to the observable drop in the crystallization efficiency. The standard deviation of each data point is presented as grey bars.

Journal logoJOURNAL OF
APPLIED
CRYSTALLOGRAPHY
ISSN: 1600-5767
Volume 53| Part 5| October 2020| Pages 1169-1180
https://doi.org/10.1107/S1600576720010687
Follow J. Appl. Cryst.
Sign up for e-alerts
E-alerts
Follow J. Appl. Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS